Figure 9

Mapping of the binding site of the human MBP peptide in CaM to the C-terminal lobe. It should be noted that the structures shown are extrapolations from the low-resolution SAXS model obtained by rigid-body fitting, as described in Methods. A. CaM (magenta) and the peptide (green) are shown as ribbons, and the interacting residues, based on the NMR experiment, are shown in stick mode with yellow colour. Note how the binding site is localised mainly to the hydrophobic pocket of the C-terminal (lower) lobe. B. Stereo view of the conformation of the CaM C-terminal lobe in free CaM (pink) and in the SAXS model of the complex (light blue). The peptide is shown in yellow, and superposition has been done using the residues of the central helix (pointing towards top right). The N-terminal lobe is omitted from the figure for clarity. An animation of the predicted movement is shown in Additional file 2.