Detailed view into the hydrophobic cavity of Mip. W-162 forms the bottom of the cavity. In the solution structure of free Mip (red) Y-185 is not part of the binding pocket. Upon binding (complex structure in blue), the aromatic ring of Y-185 moves into the hydrophobic patch displacing P-193 and I-194, (highlighted in grey) to form contacts with the ligand. In the crystal structure of free Mip (green) Y-185 shows the same orientation as in the complex.