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Table 3 Key interactions in structurally characterized E2:E3 complexes

From: Interactions between the quality control ubiquitin ligase CHIP and ubiquitin conjugating enzymes

 

E2:E3 Complex [PDB:ID Code]

Interaction E2: E3

Cbl:UbcH7 [PDB:1FBV]

CNOT4:UbcH5 [PDB:1UR6]

CHIP:UbcH5 [PDB:2OXQ]

CHIP:Ubc13 [PDB:2C2V]

E2-α1: E3-L1

    

Arg:mainchain

R6:K382O precedes I383)

R5:P15O precedes L16)

R5:I235O

R7:I235O

Arg:sidechain

 

R5:M18

R5:F237 (stacking)

R7:F237 (stacking)

salt bridges

 

K4:E19/K4:E13 K8:E13

 

R14:D229

sidechain: mainchain

  

K8:F237O

K10:F237O

E2-L4/L7: E3-α/L1/L2

    

hydrophobic

P62/F63/P97/A98:I383/W408/P417/F418

P61/F62/P95/A96:L16/I45/E49/L52/P54

P61/F62/P95/A96:I235/H260/V264/P269/V270

P63/M64/P97/A98:I235/H260/R263/V264/P269/V270

E2-L4:E3-α polar

 

F62:R44 (stacking) Q92: E49

F62:R263 (stacking)

 

E2-L4:E3-α salt bridges

 

D59:R44 K68: D48/E49

  

E2-L7: E3-L2 polar

    

S-P-A:mainchain

A98N:P417O K96:F418O

S96:P54O

S94:P269O

S96:P269O

mainchain:Arg

E93O:R420

Q92O:R57

Q92O:R272

K94O:R272

  1. Key interactions observed in structures of E2:E3 complexes [22, 30, 34]. The CNOT4:UbcH5 complex structure was not solved explicitly but modeled based on NMR structures of CNOT4 and UbcH5 and on experimental restraints derived from NMR data and mutagenesis [34].