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Table 5 Estimated Tm values from NMR thermal unfolding

From: Knottin cyclization: impact on structure and dynamics

Proton

MCoTI-II

lin-MCoTI

EETI-II

Min-23

H β Cys27

133 (4)

130 (4)

127 (3)

91 (1)

H α Gly31

127 (5)

124 (9)

-

111/117 (2)

H 2,6 Phe/Tyr32

133 (8)

118 (21)

153 (26)

97 (1)

H 3,4,5 Phe/Tyr32

115 (5)

118 (7)

-

113/86 (3)

H α Cys33

152 (8)

144 (8)

-

102 (10)

  1. Mean Tm values in °C, obtained from hundred calculations using randomly picked chemical shifts within a -0.02/+0.02 range around the experimental values, are reported. Standard deviations are shown within parentheses.
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BMC Structural Biology

ISSN: 1472-6807

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