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Table 5 Estimated Tm values from NMR thermal unfolding

From: Knottin cyclization: impact on structure and dynamics

Proton MCoTI-II lin-MCoTI EETI-II Min-23
H β Cys27 133 (4) 130 (4) 127 (3) 91 (1)
H α Gly31 127 (5) 124 (9) - 111/117 (2)
H 2,6 Phe/Tyr32 133 (8) 118 (21) 153 (26) 97 (1)
H 3,4,5 Phe/Tyr32 115 (5) 118 (7) - 113/86 (3)
H α Cys33 152 (8) 144 (8) - 102 (10)
  1. Mean Tm values in °C, obtained from hundred calculations using randomly picked chemical shifts within a -0.02/+0.02 range around the experimental values, are reported. Standard deviations are shown within parentheses.