Table 1 Representative MK2 expression constructs.

N- and C-terminal variations

MK2(36–400, K93R)

MK2(41–364)

MK2(47–357)

MK2(47–366)

Entropy-reducing surface mutations

MK2(41–364, K64A)

MK2(41–364, K343A, E344A)

MK2(47–366, K84A)

MK2(47–366, E88A, K89A)

Activation loop deletions

MK2(41–364, Δ(L220-G238))

MK2(47–366, Δ(L220-G238))

Pseudoactivation mutations

MK2(41–364, T222E)

MK2(41–364, T334E)

MK2(41–364, T222E, T334E)

MK2(47–366, T222E)

MK2(47–366, T334E)

MK2(47–366, T222E, T334E)