Table 4 Customized MK2 robotic crystallization screen conditions.
From: Rational mutagenesis to support structure-based drug design: MAPKAP kinase 2 as a case study
Variable | Parameters |
|---|---|
Buffer | Citrate HEPES |
pH | 7.0 – 8.5 |
Precipitant | Ammonium Sulfate Ammonium Malonate Na Malonate K Malonate Na/K Phosphate |
Additives | DMSO 2-Propanol PEG 400 PEG 3350 Na Malonate Na Citrate |