From: Rational mutagenesis to support structure-based drug design: MAPKAP kinase 2 as a case study
Form | Construct(s) | Crystallization Conditions * | Space Group | Unit Cell (a, >b, c, Å) | Res. (Å) N†| Notes |
---|---|---|---|---|---|---|
I | MK2(41–364) & MK2(47–366) surface mutants ‡ | AS, LS, & NP pH 5–8 | N.D. | N.D. | >11 N.D. | Rods; high mosaicity |
II | MK2(41–364) & MK2(47–366) surface mutants ‡ | AS, LS, & NM pH 5–8 | N.D. | N.D. | >11 N.D. | Plates; high mosaicity |
III | MK2(47–366) | 2 M AS 0.1 M Na citrate pH 5.0 4% 1,4-butanediol | P 213 | 215 | 3.4 4 | Cubes |
IV | MK2(47–366, T222E) | 2 M NM pH 5.5 10 μM Anapoe 80 | F 4132 | 254 | 2.9 1 | Bipyramids; inhibitor soaking req'd |
V | MK2(41–364) | 1.5–1.8 M NM pH 8.0 | P 63 | 158 158 138 | 2.6–3.3 4 | Hex. bullets; co-crystals |
VI | MK2(41–364) | 1.5–1.8 M NM pH 8.0 | P 63 | 144 144 152 | 2.6–3.3 4 | Hex. bullets; co-crystals; "collapsed" Form V |
VII | MK2(41–364) | 1.75 M AS 0.1 M Na citrate pH 8.0 | P 212121 | 140 180 215 | 2.6–3.3 12 | Sharp blocks; co-crystals |