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Figure 8 | BMC Structural Biology

Figure 8

From: Conservation of structure and activity in Plasmodium purine nucleoside phosphorylases

Figure 8

Fitting of initial velocities to different kinetic treatment methods for catalysis of 2'-deoxyinosine by Pv PNP. The left (A) shows that application of the substrate activation equation (equation 2 in the text), v = ((Vmax1·[S]) + (Vmax2·[S]2/KM2))/(KM1 + [S] + ([S]2/KM2)), (-) matches the experimental data better than does the theoretical curve from the substrate inhibition equation (---). The reciprocal plot, 1/v versus 1/[S], with ordinates divided by a factor of 500 (right, B) illustrate the upward curvature at high substrate concentration range, which is similar to an example of a substrate activation case given by [30].

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