Figure 5

Multiple structural alignment of β-propeller blades containing Asp-box repeats. This alignment was made using all blades with an Asp-box from ten representative structures: Sortilin from man (3f6k), cytoplasmic sialidase Neu2 from man (1so7), sialidase from S. typhimurium (3sil), sialidase from M. viridifaciens (1w8o), intramolecular trans-sialidase from the leech M. decora (2sli), trans-sialidase from the trypasnosome parasite T. cruzi (1ms9), endosialidase from bacteriophage K1F (1v0e), sialidase from V. cholera (1w0p), cellobiohydrolase from the fungus Geotrichum sp. M128 (1sqj; 1sqj-1 and 1sqj-2 refer to the first and second propeller respectively), and YP_299179.1 from Ralstonia eutropha (3b7f). Numberings are according to blade positions i.e. position 1 is the first position in the given blade rather than in the protein in which it occurs. The color scheme is Clustal X. The position of the Asp-box is marked by a red line, and in order to give an impression of the general localization of strands, black arrows representing the four strands of the sixth blade of 3b7f are shown beneath the alignment.