Graphical overview of the method. Graphic overview of the method used in training of the primary and secondary neural networks. 'PSSM' is a Position-Specific Scoring Matrix. 'Sec. Structure' is the raw output from secondary structure predictions. 'Primary Networks' are an ensemble of artificial neural networks (ANN) and 'B/E Classification' is the raw buried/exposed output from these ANNs. 'Secondary Networks' are also an ensemble of ANNs, trained to predict the relative surface exposure of an amino acid. The last box shows output from the web server.