The relative torsion angle between the JH1 C-helix and the JH2 C-helix. This angle is defined as the dihedral angle between the carbonyl carbon atoms of residues R893, E900, S599, and E592. Data across the bottom of the figure indicate the difference in torsion of each mutant relative to the average torsion of the wild-type JAK2 simulation (-5.71 degrees). Mutations with larger relative torsion angles exhibit greater deviation from wild-type JAK2 and are thus more likely to be deleterious. The error bars are the standard deviations of the calculated values from the last 30-ns trajectory for each mutant. Entries were obtained with a sampling frequency of 10 ps; i.e., 3,000 sample data points for each mutant simulation.