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Table 2 Gel filtration analysis of xenavidin and avidin. The samples were in the presence (+BTN) or absence (-BTN) of biotin.

From: Structural and functional characteristics of xenavidin, the first frog avidin from Xenopus tropicalis

Protein

Observed molecular weight (kDa)

Theoretical molecular weightb(kDa)

Xenavidin (-BTN)

74.5a

59.5

Xenavidin (+BTN)

73.3a

59.7

Avidin (-BTN)

62.5a

57.4

  1. aThe analyzed proteins were most probably glycosylated; sugar moieties comprise at least 10% of the mass of avidin [69].
  2. bTheoretical values were calculated from the primary structures.