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Table 1 Specific activity of wt-Trpase and its mutants at 25°C and after cooling to 2°C in Tricine -KCl buffer, pH = 7.5.

From: Conformational changes and loose packing promote E. coli Tryptophanase cold lability

Trpase

Specific activity

25°C

(Units/mg)

2°C

wt

45.7 ± 2.5

29.7 ± 1.7

W330F

42.8 ± 3.2

5.0 ± 1.4

C298S

36.9 ± 2.9

3.7 ± 0.8

Y74F*

0.2 ± 0.05

0.08 ± 0.03

  1. *The concentrations of the wt Trpase and of W330F, C298S mutants were 0.3-0.5 mg/ml, while the concentration of the Y74F mutant was 10-20 mg/ml.