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Table 3 The effect of cooling on the dissociation of wt Trpase and its mutants to dimers.

From: Conformational changes and loose packing promote E. coli Tryptophanase cold lability

  Degree of dissociation to dimers (%)
E. coli Trpase 25°C 2°C
wt holo <3 20 ± 1
C298S holo <5 64 ± 1
W330F holo <5 61 ± 2
Y74F holo 17 ± 3 66 ± 4
wt apo 70 ± 5 87 ± 3
C298S apo >80 88 ± 1
W330F apo 69 ± 4 90 ± 1
Y74F apo 80 ± 1 83 ± 2
  1. For the holo forms, the PLP concentration in Tricine-KCl buffer is 0.1 mM.