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Table 3 The effect of cooling on the dissociation of wt Trpase and its mutants to dimers.

From: Conformational changes and loose packing promote E. coli Tryptophanase cold lability

 

Degree of dissociation to dimers (%)

E. coli Trpase

25°C

2°C

wt holo

<3

20 ± 1

C298S holo

<5

64 ± 1

W330F holo

<5

61 ± 2

Y74F holo

17 ± 3

66 ± 4

wt apo

70 ± 5

87 ± 3

C298S apo

>80

88 ± 1

W330F apo

69 ± 4

90 ± 1

Y74F apo

80 ± 1

83 ± 2

  1. For the holo forms, the PLP concentration in Tricine-KCl buffer is 0.1 mM.