Figure 3

Partial structure-based sequence alignment of the β barrel fold of domain IV (D4). The aligned sequences are from B. stearothermophilus IF2, M. thermoautotrophicum aIF5B, C. thermophilum eIF5B and S. cerevisiae eIF5B with domain II (D2) from E. coli EF-Tu and S. solfataricus aIF2γ (GenBank: CAA27987, AAB84765, EGS21143, AAC04996, CAA40370, AAK40740, respectively). Highly conserved residues are highlighted in dark blue, conserved residues in light blue and similar residues in grey. Sequence numbering and secondary structure elements correspond to the ct eIF5B structure (PDB: 4N3G). As there is no structure of sc eIF5B domain IV available so far, its sequence was aligned directly with that of ct eIF5B.