Figure 4

Recognition of the methionylated 3′ CCA-end by eIF5B domain IV. A) CCA arm, A76 and the methionly moiety of Met-tRNA_i modeled into the two surface pockets on eIF5B domain IV according to the cryo-EM density. Residues implicated in the interactions with the 3′ end are indicated. B) Simulated annealing f_o-f_c omit map for the putative methionyl-binding pocket in eIF5B domain IV (blue mesh; contoured at 3σ). C) Met-tRNA_i bound to aIF2γ (PDB: 3V11). D) Phe-tRNA^Phe bound to EF-Tu (PDB: 1TTT).