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Figure 7 | BMC Structural Biology

Figure 7

From: Structural insight into the recognition of amino-acylated initiator tRNA by eIF5B in the 80S initiation complex

Figure 7

Translational β barrel fold and OB-fold share the same fold related ligand-binding interface. A and C) The interaction interface between the 3′ CCA-end of the tRNA and the translational β barrel (here Phe-tRNAPhe (purple) bound to T. aquaticus EF-Tu domain II (PDB: 1TTT)) centers on β strands 1, 2 and 5 and is augmented by loops L1, L4 and L5, containing helix αL5. B and D) A related ligand-binding interface is found in single-stranded nucleic acid binding OB-folds (here the anticodon binding domain of the aspartyl-tRNA synthetase (PDB: 1ASZ) from S. cerevisiae[35]). Similar to the interactions observed for EF-Tu and despite a different topology, the bases of the anticodon stem loop (purple) point toward the surface of the β barrel, centered on β strands 1, 2 and 3, while the flexible loops L1, L4 and L3 with αL3 form additional contacts to the phosphate backbone.

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