Figure 1

Neighbor-joining tree comparing full length amino acid sequences of PDC-related proteins. The optimal tree with the sum of branch length = 8.50849307 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) is shown next to the branches [32]. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Poisson correction method [33] and are in the units of the number of amino acid substitutions per site (scale bar). The analysis involved 31 amino acid sequences. All ambiguous positions were removed for each sequence pair. There were a total of 633 positions in the final dataset. GdPDC - G. diazotrophicus (KJ746104); GoPDC - G. oxydans (KF650839); ApPDC Acetobacter pasteurianus (AF368435.1); ZpPDC - Z. palmae (AF474145); ZmPDC - Z. mobilis (AB359063); ZmPDC - Z. mays (X17555); ScPDC - S. cerevisiae (X04675); SvPDC - S. ventriculi (AF354297); Lyngbya aestuarii (WP023067698); Acidomonas methanolica (GAJ29946); Acetobacter pomorum (WP006115789); Acetobacter aceti (WP010667855); Microcystis aeruginosa (WP_0027648); Moorea producens (WP008180762); Microbulbifer variabilis (WP020414286); Legionella pneumophila (YP006505162); MDM (CBI10829); Ktedonobacter racemifer (WP007922190); Komagataeibacter oboediens (WP010515737); Komagataeibacter hansenii (WP003622049); Komagataeibacter europaeus (WP010509054); Granulicella tundricola (YP004210504); Gluconobacter thailandicus (WP007283613); Gluconobacter morbifer (WP008852112); Gluconobacter frateurii (WP023941876); Gluconacetobacter xylinus (AHI26557); Gluconacetobacter medellinensis (YP004868149); Fluoribacter dumoffii (WP010654974); Enterobacter cloacae iPDC (P23234); Commensalibacter intestini (WP008853550); Beijerinckia indica (YP001834435); Pseudomonas putida BFD (YP008115845) ; MDM- Mine Drainage Metagenome (CBI10829.1).