Table 1 Characterization data (Steady state kinetic constants, T opt and pH opt ) for GdPDC using pyruvate and compared with those from other Gram negative bacteria (The values represent the average of at least two individual rounds of protein purification and assay)
PDC | K M (mM) | Specific activity in (U/mg) | k cat /K M (M−1.s−1) | Topt(°C) | T½at °C | pHopt |
|---|---|---|---|---|---|---|
GdPDC | 0.06 (5.0)* | 20 (5.0) | 1.3 × 106 (5.0) | 45-50 | 18 min at 60°C | 5.0-5.5 |
0.60 (6.0) | 39 (6.0) | 2.6 × 105 (6.0) | ||||
1.2 (7.0) | 43 (7.0) | 1.4 × 105 (7.0) | ||||
GoPDC | 0.12 (5.0) [[7]] | 57 (5.0) [[7]] | 1.9 × 106 (5.0) [[7]] | 53 [[7]] | 10 min at 65°C [[7]] | 4.5-5.0 [[7]] |
1.2 (6.5) [[7]] | 47 (6.5) [[7]] | 1.6 × 105 (6.5) [[7]] | ||||
2.8 (7.0) [[7]] | 125 (7.0) [[7]] | 1.8 × 105 (7.0) [[7]] | ||||
ApPDC | 1.3 × 106 (5.0)#[[5]] | 65 [[36]] | 24 min at 70°C [[36]] | 3.5 - 6.5 [[36]] | ||
ZpPDC | 1.4 × 106(6.0)#[[5]] | 55 [[36]] | 24 min at 60°C [[36]] | 7.0 [[36]] | ||
ZmPDC | 1.3 (6.5) [[36]]/0.31 (6.0) [[26]]/1.1 [[37]]/0.4 (6.0) [[38]] | 1.9 × 106 (6.0) [[26]] / 4.4 × 105 (6.5) [[37]]/ 1.79 × 106 (6.0) [[38]] | 60 [[36]] | 30 min at 60°C [[36]] | 6.0-6.5 [[36]] | |
SvPDC | 13 [[6]] | 103 [[6]] | 3.2 × 104[[6]] / 0.87 × 104 (7.0) | N/A | 30 min at 50°C | 6.3 - 6.7 [[6]] |