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Table 1 Characterization data (Steady state kinetic constants, T opt and pH opt ) for GdPDC using pyruvate and compared with those from other Gram negative bacteria (The values represent the average of at least two individual rounds of protein purification and assay)

From: Structure and functional characterization of pyruvate decarboxylase from Gluconacetobacter diazotrophicus

PDC K M (mM) Specific activity in (U/mg) k cat /K M (M−1.s−1) Topt(°C) T½at °C pHopt
GdPDC 0.06 (5.0)* 20 (5.0) 1.3 × 106 (5.0) 45-50 18 min at 60°C 5.0-5.5
0.60 (6.0) 39 (6.0) 2.6 × 105 (6.0)
1.2 (7.0) 43 (7.0) 1.4 × 105 (7.0)
GoPDC 0.12 (5.0) [[7]] 57 (5.0) [[7]] 1.9 × 106 (5.0) [[7]] 53 [[7]] 10 min at 65°C [[7]] 4.5-5.0 [[7]]
1.2 (6.5) [[7]] 47 (6.5) [[7]] 1.6 × 105 (6.5) [[7]]
2.8 (7.0) [[7]] 125 (7.0) [[7]] 1.8 × 105 (7.0) [[7]]
ApPDC 2.8 (6.5) [[36]]/0.39 (5.0) [[5]] 110 (6.5) [[36]]/97 (5.0) [[5]] 1.3 × 106 (5.0)#[[5]] 65 [[36]] 24 min at 70°C [[36]] 3.5 - 6.5 [[36]]
ZpPDC 2.5 (6.5) [[36]]/0.24 (6.0) [[5]] 116 (6.5) [[36]]/130 (6.0) [[5]] 1.4 × 106(6.0)#[[5]] 55 [[36]] 24 min at 60°C [[36]] 7.0 [[36]]
ZmPDC 1.3 (6.5) [[36]]/0.31 (6.0) [[26]]/1.1 [[37]]/0.4 (6.0) [[38]] 120 (6.5) [[36]]/120 [[37]]/181 [[38]] 1.9 × 106 (6.0) [[26]] / 4.4 × 105 (6.5) [[37]]/ 1.79 × 106 (6.0) [[38]] 60 [[36]] 30 min at 60°C [[36]] 6.0-6.5 [[36]]
SvPDC 13 [[6]] 103 [[6]] 3.2 × 104[[6]] / 0.87 × 104 (7.0) N/A 30 min at 50°C 6.3 - 6.7 [[6]]
  1. Numbers after values are the references from which the numbers were obtained.
  2. *Values in brackets indicate assay pH.
  3. #Calculated based on values given in reference [5].