HMTase activities of NSD1-CTD, NSD2-CTD, and NSD3-CTD on H3 and H4 substrates in vitro. HMTase activities of NSD1-CTD, NSD2-CTD, and NSD3-CTD on H3K4, K9, K27, K36, K79, and H4K20 were measured using colorimetric quantification (See Materials and Methods). HMTase assays were done in duplicate individual experiments and all samples were in duplicate. The error bars indicate standard deviations. The results were normalized against controls that did not contain any enzymes (NC, 0 μg). Asterisks indicate P-values; *p < 0.05, **p < 0.02, ***p < 0.005, ****p < 0.001.