Table 3 Kinetics and affinity constants for RGD-hirudin and mutant variant binding to thrombin
Variant analyzed | Association rate ka(M−1 s−1) | Dissociation rate kd(s−1) | Binding affinity KD(M) |
|---|---|---|---|
RGD-hirudin | 1.77 × 103 | 2.73 × 10−5 | 1.55 × 10−8 |
Y3A | 0.80 | 2.88 × 10−3 | 3.61 × 10−3 |
S50A | 1.12 × 103 | 3.94 × 10−5 | 3.50 × 10−8 |
Q53A | 59.20 | 3.37 × 10−5 | 5.70 × 10−7 |
D55A | 371.00 | 3.83 × 10−4 | 1.03 × 10−6 |
Q57A | 155.00 | 3.74 × 10−4 | 2.41 × 10−6 |
I59A | 73.00 | 1.39 × 10−3 | 1.90 × 10−5 |