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Table 3 Kinetics and affinity constants for RGD-hirudin and mutant variant binding to thrombin

From: Structural basis of RGD-hirudin binding to thrombin: Tyr3 and five C-terminal residues are crucial for inhibiting thrombin activity

Variant analyzed Association rate ka(M−1 s−1) Dissociation rate kd(s−1) Binding affinity KD(M)
RGD-hirudin 1.77 × 103 2.73 × 10−5 1.55 × 10−8
Y3A 0.80 2.88 × 10−3 3.61 × 10−3
S50A 1.12 × 103 3.94 × 10−5 3.50 × 10−8
Q53A 59.20 3.37 × 10−5 5.70 × 10−7
D55A 371.00 3.83 × 10−4 1.03 × 10−6
Q57A 155.00 3.74 × 10−4 2.41 × 10−6
I59A 73.00 1.39 × 10−3 1.90 × 10−5