Skip to main content

Table 1 Kinetic parameters for 5α-DHT reduction measured by spectrophotometer

From: Structural importance of the C-terminal region in pig aldo-keto reductase family 1 member C1 and their effects on enzymatic activity

Enzyme

V max

K m

K cat

K cat /K m

(nmol/min per mg)

(μM)

(s −1 )

(s −1  M −1 )

AKR1C1 C-type

25.74 ± 2.338

4.978 ± 3.091

0.011 ± 0.000999

2,210 ± 323.196

AKR1C1 T-type

75.5 ± 5.2

7.736 ± 2.816

0.0340 ± 0.00234

4,340 ± 830.966

  1. Each value indicates mean ± SEM (n = 3).
  2. Kinetic parameters were determined using data in Figure 1 through GraphPad prism 6 software (GraphPad Software Inc., San Diego, CA).
Back to article page

BMC Structural Biology

ISSN: 1472-6807

Contact us