Figure 3

Structure and sequence comparisons between Lb NDK and Lm NDK. (A) CLUSTALW alignment of the C-terminal sequence from LmNDK and LbNDK. The region not modelled in the LbNDK crystal structure (gray) contains only two amino acids residues (Ala¹⁴⁶ and Cys¹⁴⁷) that are not conserved in LmNDK (Val¹⁴⁶ and Ser¹⁴⁷). (B) Cartoon representation of LbNDK structure superimposed in the LmNDK structure highlighting the C-terminal extension partially unfolded in LbNDK (modelled up to residue 142 and colored in yellow) and fully folded in LmNDK (purple). In sticks (purple) are residues that form a hydrophobic cluster that stabilize the C-terminal extension of LmNDK and Ser¹⁴⁷ that is substituted by a cysteine in LbNDK.