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Table 2 Structural and hydrodynamic parameters calculated from DLS and SAXS experiments under different pH conditions

From: Crystal structure and biophysical characterization of the nucleoside diphosphate kinase from Leishmania braziliensis

 

pH

4.0

5.0

6.0

7.0

8.0

9.0

DLS

R h (Ă…)

46

46

44

44

44

45

 

MW (kDa)

120

117

106

108

109

106

 

% Pd

12.6

10.1

12

11.5

11.4

10.8

SAXS

R g (Ă…)

34

33

32

31

-

-

 

D max (Ă…)

86

85

83

84

-

-

 

MW (kDa)

110

110

109

109

-

-

  1. R h is the hydrodynamic radius, MW is the calculated molecular weight, % Pd corresponds to the sample polydispersity, R g is the radius of gyration estimated by the Guinier approximation and D max is the maximum molecule diameter estimated from the pair-distance distribution function P(r).
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BMC Structural Biology

ISSN: 1472-6807

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