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Table 2 Structural and hydrodynamic parameters calculated from DLS and SAXS experiments under different pH conditions

From: Crystal structure and biophysical characterization of the nucleoside diphosphate kinase from Leishmania braziliensis

  pH 4.0 5.0 6.0 7.0 8.0 9.0
DLS R h (Å) 46 46 44 44 44 45
  MW (kDa) 120 117 106 108 109 106
  % Pd 12.6 10.1 12 11.5 11.4 10.8
SAXS R g (Å) 34 33 32 31 - -
  D max (Å) 86 85 83 84 - -
  MW (kDa) 110 110 109 109 - -
  1. R h is the hydrodynamic radius, MW is the calculated molecular weight, % Pd corresponds to the sample polydispersity, R g is the radius of gyration estimated by the Guinier approximation and D max is the maximum molecule diameter estimated from the pair-distance distribution function P(r).