Figure 1
From: High-resolution structure of a type IV pilin from the metal-reducing bacterium Shewanella oneidensis
Sequence alignment and construct of Pil Bac1 . A: Sequence alignment of the N-terminal part of PilBac1 (PilBac1NT) with the N-terminal parts of type pilins and pseudopilins. Type IV pilins: PilE from N. gonorrhoeae, PilA from P. aeruginosa; PilA from D. nodosus, PilA from G. sulfurreducens.; Pseudopilins: XcpT from P. aeruginosa; EpsG from V. cholerae; EtpG from E. coli; PulG from K. oxytoca. The protease cleavage site is marked with an arrow and the hydrophobic transmembrane helix is framed in red and the residues introducing kink2 in blue. The alignment was performed with the program MUSCLE installed in CLC Genomics Workbench 6.9.1 [53]. B: Construct of PilBac1∆N. SP: leader sequence; TM HELIX: transmembrane α-helix; His6: 6 residue long Histidine tag; EK: enterokinase cleavage site; TEV: TEV protease cleavage site. The aromatics and the cysteines are highlighted in red and blue in the sequence respectively.