Table 2 Cloning primers used in this work
Name | Sequence |
|---|---|
Fw1 | 5′-AAAAAGCAGGCTTCGAAGGAGATAGAACCATGGTGATGAAAGCCAAT GTCACC |
Rev1 | 5′-AGAAAGCTGGGTCTTAGTGATGGTGATGGTGATGTCCCTGGAAATACA GGTTTTCCGCGAACTCTGGCCTCGGTTC |
Rev2 | 5′-AGAAAGCTGGGTCTTAGTGATGGTGATGGTGATGTCCCTGGAAATACA GGTTTTCAACGCGCAGCGTGACCATGACC |
attB1 adapter | 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCT |
attB2 adapter | 5′-GGGGACCACTTTGTACAAGAAAGCTGGGT |
Ins-fw | 5′-CATTTTCCGCGCCCGTAGGTGGGGGA |
Ins-rev | 5′-TCCCCCACCTACGGGCGCGGAAAATG |