The workflow for evolutionary interaction pattern derivation up to final structure similarity determination.
A: The main process to derive family-specific EIPP records. This includes the protein data aggregation from known membrane protein structures and the detection of evolutionary covariation based on pattern alignments (PAs). Together with interaction data information from TMPad , we obtain interacting patterns with evolutionary background, which are important for maintaining structure and function. B: The evaluation process includes to obtain α-helical sequence information from unknown membrane protein structures using by TMHMM [6,7]. Finally, signature EIPPs can be searched in unknown structures with final structure similarity determination to known structures.