Data collection | EMPa derivative | Native |
---|---|---|
Space group | R32 | R32 |
Number of monomers per asymmetric unit | 1 | 1 |
Unit cell dimensions | ||
a, b, c (Å) | 126.8, 126.8, 105.7 | 130.0, 130.0, 105.2 |
α, β, γ (°) | 90, 90, 120 | 90, 90, 120 |
Resolution (Å) | 50.0-3.1 (3.2-3.1)b | 50.0-3.1 (3.15-3.10) |
I/σ | 28 (1.9) | 34.3 (2.6) |
Completeness (%) | 98.1 (92.8) | 99.7 (98.3) |
Redundancy | 3.4 (3.2) | 7.1 (5.4) |
Rmeas | 0.046 (0.571) | 0.077 (0.697) |
C1/2 c in the highest resolution shell | 0.76 | 0.89 |
Number of unique reflections | 11759 (1102) | 6322 (296) |
Structure refinement statistics | ||
Resolution (Å)d | 25.0-3.4 | |
R (%) | 32.5 | |
Rfree (%) | 33.3 | |
Number of non-hydrogen atoms | 2194 | |
Bond length deviation (rmsd) from ideal (Å) | 0.009 | |
Bond angle deviation (rmsd) from ideal (°) | 1.44 | |
Clashscore | 6 | |
Ramachandran plot statisticse | ||
% residues in allowed regions | 93.7 | |
% residues in generously allowed regions | 3.9 | |
% residues in disallowed regions | 2.4 |