Table 1 Bacterial strains used in this study
From: New insights into the molecular mechanism of the Rab GTPase Sec4p activation
Strain number | Bacterial strain | Description | Vector used |
|---|---|---|---|
RCB4554 | BL21 (DE3) | Sec4pV29 (19–187) | ppSUMO |
RCB4699 | BL21 (DE3) | Sec4pWT (19–187) | ppSUMO |
RCB4636 | BL21 (DE3) | Sec2p (51–142) | ppSUMO |
RCB4727 | BL21 (DE3) | Sec2p (51–182) | ppSUMO |
RCB5290 | BL21 (DE3) | Sec2p (51–160) | ppSUMO |
RCB5291 | BL21 (DE3) | Sec2 (1–160) | ppSUMO |
RCB5117 | DH5α | Sec2p (51–182) mutant 153–157Ala | ppSUMO |
RCB5119 | DH5α | Sec2p (51–182) mutant 142–147Ala | ppSUMO |
RCB5121 | DH5α | Sec2p (51–182) mutant F109A | ppSUMO |
RCB5214 | BL21 (DE3) | Sec4p (19–187) mutant E80A and R81A, | ppSUMO |
RCB5215 | BL21 (DE3) | Sec2p (51–142) K140C, | ppSUMO |