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Table 1 Bacterial strains used in this study

From: New insights into the molecular mechanism of the Rab GTPase Sec4p activation

Strain number Bacterial strain Description Vector used
RCB4554 BL21 (DE3) Sec4pV29 (19–187) ppSUMO
RCB4699 BL21 (DE3) Sec4pWT (19–187) ppSUMO
RCB4636 BL21 (DE3) Sec2p (51–142) ppSUMO
RCB4727 BL21 (DE3) Sec2p (51–182) ppSUMO
RCB5290 BL21 (DE3) Sec2p (51–160) ppSUMO
RCB5291 BL21 (DE3) Sec2 (1–160) ppSUMO
RCB5117 DH5α Sec2p (51–182) mutant 153–157Ala ppSUMO
RCB5119 DH5α Sec2p (51–182) mutant 142–147Ala ppSUMO
RCB5121 DH5α Sec2p (51–182) mutant F109A ppSUMO
RCB5214 BL21 (DE3) Sec4p (19–187) mutant E80A and R81A, ppSUMO
RCB5215 BL21 (DE3) Sec2p (51–142) K140C, ppSUMO