Fig. 8

Bio-layer Interferometry. Association of SpCBM with 3’SL immobilized to a probe via a biotin linkage as measured using bio-layer interferometry. a Sensogram showing the response units against increasing concentrations of SpCBM. b Steady-state binding of SpCBM-3’SL interaction determined by plotting the response at equilibrium against SpCBM concentration and globally fitted using a 1:1 (Langmuir) binding model