Skip to main content
Fig. 3 | BMC Structural Biology

Fig. 3

From: An intact helical domain is required for Gα14 to stimulate phospholipase Cβ

Fig. 3

Role of β2-β3 and α2-β4-α3 regions of Gα14 in interaction and activation of PLCβ. a Schematic representation of zα2β4α3, 14α2β4α3, zβ2β3 and 14β2β3 chimeras. b, Cells were co-transfected with PLCβ2 and Gα protein or the indicated chimeras. Co-immunoprecipitation assays were performed and analyzed as in Fig. 2. Data shown represent one of three sets of immunoblots; two other sets yielded similar results. c HEK293 cells were transiently transfected with the wild-type or constitutively active mutants (QL) of Gα proteins or chimeras and then subjected to IP3 accumulation assay and analyzed as in Fig. 2. *, IP3 production was significantly enhanced as compared to corresponding wild-type transfected cells; Dunnett t test, p < 0.05

Back to article page