Fig. 2

HAZV N purification by ion exchange chromatography. HAZV N was separated from GST-HAZV N and GST using ion exchange chromatography. a Protein elution was monitered with an A₂₈₀ absorbance trace; protein that flowed through the column without binding is represented by Peak 1, whereas protein that bound to the column and was then eluted by an increasing NaCl gradient (dashed grey line) forms Peak 2. b SDS PAGE analysis of Peak 1 and Peak 2. Peak 2 contains primarily HAZV N, whereas Peak 1 contains a mixture of proteins that flowed through the column without binding