Fig. 3

Circular dichroism analysis of CCHFV N and HAZV N. Circular Dichroism was used to analyse the secondary structure of both HAZV and CCHFV N proteins. a Ellipticity across the far UV spectra illustrate that both proteins are primarily α – helical, demonstrated by the characteristic minima at 208 nm and 222 nm. b The thermal stability of CCHFV and HAZV N proteins were compared by monitoring ellipticity at 222 nm, we find that the melting temperature (Tm) of HAZV N is 42.21 °C and of CCHFV N is 46.30 °C