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Fig. 3 | BMC Structural Biology

Fig. 3

From: Crystal structure of human S100A8 in complex with zinc and calcium

Fig. 3

Oligomeric states of hS100A8 in the presence of divalent cations. a Superimposition of the Zn2+/Ca2+-bound hS100A8 homodimers from crystal form 1 (beige) and crystal form 2 (cyan) with Ca2+-hS100A8 (dark blue) [14]. The major regions of divergence are indicated with red arcs. b Elution profile of hS100A8 on a 24 ml Superdex 75 size exclusion chromatography column (Ge Healthcare Life Sciences) equilibrated in 20 mM HEPES pH 7.5, 200 mM NaCl, 5 mM CaCl2. Ca2+-hS100A8 elutes as two peaks with elution volumes of 11.15 and 12.8 ml, respectively, most likely corresponding to a tetramer and a dimer, respectively. c Superimposition of the Zn2+/Ca2+-bound hS100A8 homotetramers from crystal form 1 (yellow) and crystal form 2 (cyan) with the homotetramer derived from the crystal packing in Ca2+-hS100A8 (dark blue, [14]) and with the (S100A8/S100A9)2 heterotetramer (salmon, [17]). The red arrow indicates the movement of one S100A8 homodimer away from the other homodimer on one side of the dimer-dimer interface in the calcium-loaded hS100A8 [14]. d Close-up view on the interactions stabilizing the homodimer-homodimer packing within the hS100A8 homotetramer from Ca2+-hS100A8 (purple) and from Zn2+/Ca2+-hS100A8 (yellow, crystal from 1)

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