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Fig. 2 | BMC Structural Biology

Fig. 2

From: A lack of peptide binding and decreased thermostability suggests that the CASKIN2 scaffolding protein SH3 domain may be vestigial

Fig. 2

An SH3-6x mutant (K290Y, H296E, A300E, R319W, Y336L, I341F) mimics the binding cleft of the STAM2 SH3 domain in a CASKIN2 SH3 domain framework and consequently, high affinity towards a STAM2 ligand (YTPMVRENKPP) derived from the protein, UBPY. From direct curve fitting against a binding equation describing a 1:1 protein to peptide complex, the dissociation constants (K d ) of wild type CASKIN2 SH3 domain and 6x mutant are 39 ± 6 μM and 220 ± 20 nM, respectively

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BMC Structural Biology

ISSN: 1472-6807

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