A lack of peptide binding and decreased thermostability suggests that the CASKIN2 scaffolding protein SH3 domain may be vestigial

Table 1 Restraints and statistics for the ensemble of 15 structures

NOE restraints
	Total	413
	Intraresidue (\|i – j\| = 0)	204
	Sequential (\|i – j\| = 1)	102
	Medium range (1 < \|i – j\| < 5)	8
	Long range (\|i – j\| ≥ 5)	99
Additional restraints
	Hydrogen bond distance restraints	32
	Backbone angle torsion angle restraints	74
RMS deviations^a
	Bonds	0.0123 ± 0.0003
	Angles	1.2827 ± 0.0363
	Improper angles	1.6905 ± 0.1112
	Dihedral angles	0.2015 ± 0.1411
RMS violations
	NOE restraints > 0.5 Å	0.0 ± 0.0
	NOE restraints > 0.3 Å	2.3 ± 1.2
	NOE restraints > 0.1 Å	25.5 ± 4.1
	Dihedral angles > 5°	6.2 ± 0.6
Ramachandran analysis for ordered residues^b
	Most favored regions	95.2 %
	Additional allowed regions	4.8 %
	Generously allowed regions	0.0 %
	Disallowed regions	0.0 %

ISSN: 1472-6807