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Fig. 2 | BMC Structural Biology

Fig. 2

From: Prokaryotic ubiquitin-like protein remains intrinsically disordered when covalently attached to proteasomal target proteins

Fig. 2

Preparative pupylation of MtbFabD-3KR (K35R, K122R and K181R) and MtbPanB with 15N-labeled MtbPup. Pupylation of 40 μM MtbPanB or 40 μM MtbFabD-3KR with 35 μM 15N-MtbPup in the presence of 10 mM ATP and 2.5 μM CgluPafA-His6 in 2500 μl total reaction volume and subsequent removal of the Pup ligase using a Ni-NTA spin-column (FT: flowthrough, E: elution with 250 mM Imidazole)

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BMC Structural Biology

ISSN: 1472-6807

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