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Fig. 5 | BMC Structural Biology

Fig. 5

From: Prokaryotic ubiquitin-like protein remains intrinsically disordered when covalently attached to proteasomal target proteins

Fig. 5

CgluPup covalently attached to a substrate remains accessible to CgluPafA and binds to it with similar affinities as free CgluPup. CgluPafA: CgluPup ~ substrate affinity measurements using fluorescence anisotropy with a fluorescein-labeled CgluPup-Q30C variant. a Affinity of CgluPafA for CgluPup ~ MtbFabD-3KR (green) or b CgluPup ~ MtbPanB (blue) lies in a similar range as the affinity for free CgluPup-Q30C (red, a and b)

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BMC Structural Biology

ISSN: 1472-6807

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