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Table 3 Predicted and measured changes in heat stability for various P2 variants. Tm values for P38G and the disease mutations have been published [11, 25]

From: Structure and dynamics of a human myelin protein P2 portal region mutant indicate opening of the β barrel in fatty acid binding proteins

variant Cupsat ∆∆G (kcal/mol) MAESTRO ∆∆G (kcal/mol) Experimental ∆Tm (°C)
F57A −1.91 1.16 −6
P38G −5.38 1.64 −6
I43N −7.63 3.92 −17
T51P −1.51 1.43 −24
I52T −5.17 2.89 −13
  1. Note that Cupsat defines a destabilizing mutation with a negative ∆∆G, while in MAESTRO, a destabilizing mutation has a positive ∆∆G