Fig. 3

Determination of the times for stopping the simulation and of the possible onset of overfitting. The simulation proceeds until point A, where the estimate of the stopping time (red curve) becomes equal to the current time (blue line). At that point, the estimate of the onset of overfitting is given by the orange curve (point B: warning time). (This plot corresponds to the lactoferrin test case. See Fig. 4, where the value t₁=53 (warning time, see “Methods” Section) obtained from this plot is indicated.)