Table 1 Primers used in this study
From: Crystal structure of carbonic anhydrase CaNce103p from the pathogenic yeast Candida albicans
Construct | Primer name | Sequence | Restriction site |
|---|---|---|---|
WT | WT_CaNCE103 Nde I F | AAGGAGATATACATATGATGGGTAGAGAAAATATTTTGAA | NdeI |
Δ29/Δ48/Δ61_CaNCE103 Xho I R | GGTGGTGGTGCTCGAGTCAATGAGGGTTATATTCTTCTTC | XhoI | |
Δ29 | Δ29_CaNCE103 Nde I F | AAGGAGATATACATATGGATAATAATAACAACCTAAACGG | NdeI |
Δ29/Δ48/Δ61_CaNCE103 Xho I R | GGTGGTGGTGCTCGAGTCAATGAGGGTTATATTCTTCTTC | XhoI | |
Δ48 | Δ48_CaNCE103 Nde I F | AAGGAGATATACATATGGTTAGTTCAGGAAATCATAATAAT | NdeI |
Δ29/Δ48/Δ61_CaNCE103 Xho I R | GGTGGTGGTGCTCGAGTCAATGAGGGTTATATTCTTCTTC | XhoI | |
Δ61 | Δ61_CaNCE103 Nde I F | AAGGAGATATACATATGTCTTCAGAATCTACATTACAAGATTT | NdeI |
Δ29/Δ48/Δ61_CaNCE103 Xho I R | GGTGGTGGTGCTCGAGTCAATGAGGGTTATATTCTTCTTC | XhoI |