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Figure 2 | BMC Structural Biology

Figure 2

From: Progesterone modulation of transmembrane helix-helix interactions between the α-subunit of Na/K-ATPase and phospholipid N-methyltransferase in the oocyte plasma membrane

Figure 2

Induction of PME formation in isolated oocyte plasma membranes membrane by exogenous progesterone, R5020 (a non-metabolizable progestin), 5α-3,20-pregnanedione, and 17β-estradiol. Plasma-vitelline membranes isolated from denuded prophase-arrested R. pipiens oocytes were preincubated with [3H]S-adenosyl methionine ([3H]SAM) for 2 min at 20°C before addition of 800 nM (final concentration) of the steroid indicated. Individual samples containing 5-6 isolated membranes were frozen in liquid nitrogen at 0, 15, 30, 60 and 120 s after progesterone addition. Membranes were then extracted and analyzed for [3H]phosphatidylmonomethylethanolamine (PME) as described in Methods. Values are means ± SEM for oocytes from 3 females.

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BMC Structural Biology

ISSN: 1472-6807

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