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Table 1 Primer sequences for constructing chimeras between Gα16 and Gαz.

From: 16 interacts with tetratricopeptide repeat 1 (TPR1) through its β3 region to activate Ras independently of phospholipase Cβ signaling

Construct

Primers:

Antisense Primer Sense Primer

Size (aa)

N102

5'-GGGCCTGCTGAATGG GATCCTGAGGGCGGC-3'

365

 

3'-CCCGGACGACTTACCCTAGGACACCCGCCG-5'

 

N155

5'-GGCTGAATCGAGCAGGTGGTACTCGCTGGA-3'

367

 

3'-CCGACTTAGCTCGTCCACCATGAGCGACCT-5'

 

N188

5'-GTTGATGCCAGTGGTCATGTCCCGGGAGCG-3'

367

 

3'-CAACTACGGTCACCAGTACAGGGCCCTCGC-5'

 

N200

5'-CCGCAGGTTGGTTTTCTTGAAGGTGAACTT-3'

367

 

3'-GGCGTCCAACCAAAAGAACTTCCACTTGAA-5'

 

N210

5'-CTCTGACTTCTGGCCCCCCACGTCCACCAT-3'

367

 

3'-GAGACTGAAGACCGGGGGGTGCAGGTGGTA-5'

 

N210QL

5'-CTCTGACTTCAGGCCCCCCACGTCCACCAT-3'

367

 

3'-GAGACTGAAGTCCGGGGGGTGCAGGTGGTA-5'

 

N246

5'-CATGCGGTTCTCCTGGTTATCCTCGTAGAG-3'

367

 

3'-GTACGCCAAGAGGACCAATAGGAGCATCTC-5'

 

N266

5'-GGATGTGCTTTTGAACCAGTTGTTGTTGCA-3'

367

 

3'-CCTACACGAAAACTTGGTCAACAACAACGT-5'

 

N295

5'-GCCCTGGAAACTGGGAAAGCAGATGGTGAG-3'

367

 

3'-CGGGACCTTTGACCCTTTCGTCTACCACTC-5'

 

C164

5'-CTCTGACCTCTGCCC CCCGACGTCCACGAT-3'

362

 

3'-GAGCAYGGAGACGGG GGGCTGCAGGTGCTA-5'

 

C164QL

5'-CTCTGACCTCAGCCC CCCGACGTCCACGAT-3'

362

 

3'-GAGCATGGAGTCGGG GGGCTGCAGGTGCTA-5'

 

C174

5'-CTTGAAGGTGAGCTC CTGCACGGAGAAGCA-3'

362

 

3'-GAACTTCCACTCGAG GACGTGCCTCTTCGT-5'

 

C186

5'-CACAATGCCCGTGGT GGGCATGCGGCTGCG-3'

362

 

3'-GTGTTACGGGCACCA CCCGTACGCCGACGC-5'

 

C219

5'-CGCGTTGTCCTCCAG GTGGAATTCCCGCCG-3'

362

 

3'-GCGCAACAGGTCCAC CACCTTAAGGGCGGC-5'

 

C272

5'-CTTGATTTCGCGGCG GTGGTCCTGCTTCTG-3'

362

 

3'-GAACTAAAGCGCCGC CACCAGGACGAAGAC-5'

 
  1. Bold and italic nucleotides denote the Gα16-derived sequences.