Construct | Intact PLCβ Domain | A 1 R-induced IP accumulation | QL-induced IP accumulation | ||
---|---|---|---|---|---|
Basal | 10 μM CHA | Fold Stimulation | Fold Stimulation | ||
pcDNA1 | N/A | 10 ± 3 | 11 ± 2 | 1.1 ± 0.2 | N/A |
Gα16 | Yes | 23 ± 5 | 237 ± 13 | 10.0 ± 0.6* | 7.8 ± 0.4 # |
N30 | Yes | 23 ± 4 | 81 ± 10 | 3.5 ± 0.7* | 6.5 ± 0.4 # |
N102 | Yes | 17 ± 4 | 13 ± 4 | 0.8 ± 0.2 | 1.1 ± 0.1 |
N155 | Yes | 13 ± 3 | 10 ± 1 | 0.8 ± 0.1 | 1.0 ± 0.1 |
N188 | Yes | 19 ± 4 | 155 ± 13 | 8.2 ± 0.3* | 4.2 ± 0.2 # |
N200 | Yes | 11 ± 3 | 15 ± 2 | 1.3 ± 0.1 | 2.0 ± 0.2 # |
N210 | Yes | 12 ± 3 | 10 ± 2 | 0.8 ± 0.2 | 1.3 ± 0.1 |
N246 | No | 10 ± 2 | 10 ± 1 | 1.0 ± 0.1 | 0.8 ± 0.0 |
N266 | No | 27 ± 3 | 17 ± 2 | 0.6 ± 0.3 | 1.2 ± 0.2 |
N295 | No | 9 ± 1 | 9 ± 1 | 1.0 ± 0.1 | 0.9 ± 0.2 |
C25 | Yes | 15 ± 3 | 92 ± 7 | 6.1 ± 0.5* | 4.1 a |
C44 | Yes | 78 ± 11 | 183 ± 15 | 2.3 ± 0.2* | 2.6 ± 0.1 # |
C164 | No | 9 ± 2 | 8 ± 3 | 0.9 ± 0.3 | 1.3 ± 0.2 |
C174 | No | 13 ± 3 | 14 ± 9 | 1.1 ± 0.1 | 1.4 ± 0.2 |
C186 | No | 4 ± 1 | 6 ± 2 | 1.5 ± 0.5 | 1.4 ± 0.2 |
C219 | No | 7 ± 1 | 9 ± 2 | 1.3 ± 0.3 | 1.2 ± 0.1 |
C272 | No | 8 ± 1 | 9 ± 1 | 1.1 ± 0.1 | 1.2 ± 0.1 |
zβ2β3 | Yes | 21 ± 5 | 51 ± 2 | 3.0 ± 0.4* | 4.3 ± 0.4 # |
zβ3 | Yes | 14 ± 1 | 79 ± 5 | 5.6 ± 0.3* | 6.9 ± 0.4 # |
N200-C164 | No | 15 ± 1 | 19 ± 1 | 1.3 ± 0.1 | 0.9 ± 0.2 |
N188-C164 | No | 13 ± 0 | 14 ± 1 | 1.1 ± 0.0 | 1.0 ± 0.2 |
Gαz | No | 10 ± 1 | 9 ± 3 | 0.9 ± 0.3 | 1.1 ± 0.1 |