Structural insights into the membrane-extracted dimeric form of the ATPase TraB from the Escherichia coli pKM101 conjugation system

Table 1 Theoretical and experimental Molecular Mass (MM) determination

	MM^Calc	GF calibration	D.L.S	BN-PAGE	Oligomeric state
TraB _FL	103	198	184	146 - 242	Dimer
TraB _NT	55	122	ND	ND	Dimer
TraB _CT	49	100	100	66 - 146	Dimer
LvhB4	97	93	105	ND	Monomer

All MM are given in kDa. "MMCalc" is the MM calculated from the amino-acid sequence, "GF" stands for gel filtration, "DLS" stands for Dynamic Light Scattering, "BN-PAGE" stands for Blue Native-PAGE. The oligomeric state is deduced from the comparison between MM^Calc and the experimental MM as determined by the other approaches. "ND" stands for non-determined.

ISSN: 1472-6807