Figure 2

Characterization of C-terminal Vpr peptides interacting with CypA. SPR sensorgrams for synthetic Vpr^75-90, the mutants Vpr^75-90 (R76Q, V83I, T84I), Vpr^75-90 (R76Q, V83I, R80A, T84I), Vpr^75-90 (R80A) and the shorter peptides Vpr^69-78, Vpr^75-84, Vpr^81-90 and Vpr^87-96 were analyzed for binding to immobilized recombinant CypA. The peptides were injected at concentrations ranging from 0-8 μM over CM5 chip immobilized to 150 RU with CypA.