Figure 5
Characterization of binding of full-length Vpr to CypA. Characterization of CypA binding to full-lengthVpr at different chip concentrations of CypA. The interaction of Vpr1-96 with immobilized recombinant CypA was analyzed using SPR biosensor system. Vpr1-96 was injected over CM5 chips immobilized with different surface densities of CypA. Comparison of binding response curves detected for Vpr1-96 injected at concentrations ranging from 0-80 nM over CM5 chip immobilized with 180 RU (A) and over chip immobilized with 918 RU CypA (B) shows an increase in response with increased CypA concentration immobilized.