Figure 8
From: Prediction of transmembrane helix orientation in polytopic membrane proteins
Conservation of residues and lipid-exposed surfaces in PetN subunit of cytochrome b 6 f. (A) Sequence logo for PetN subunit (31 sequences, pairwise sequence identity 29%–88%). (B) Highly conserved residues Trp8, Ser18, Val22, and Arg26 face phospholipids in 1VF5 structure. (C) Highly conserved residues Ser87 and Val91 are buried within the helical bundle in 1Q90 structure. Residue Trp77 forms a hydrogen bond with conserved Gln37 from the soluble domain of cytochrome f. (D) Interhelical intersubunit hydrogen bond between Arg95 from PetN and Asp35 from subunit IV in 1Q90 (only TM domain is shown for clarity).