Figure 8

Cartoon illustrating the separation and detection procedures used to investigate the interaction between E. coli and Sudan red compounds (L). (1) E. coli culture medium; (2) supernatant of (1) after centrifugation at 3500 × g; (3) E. coli collected; (4) E. coli liquid re-suspended with PBS still containing Sudan dyes (L) ; (5) supernatant of (4) after centrifugation at 3500 × g; (6) E. coli – L binding substance collected; (7) E. coli – L binding liquid re-suspended with PBS; (8) E. coli – L binding liquid after ultrasonication; (9) supernatant of (8) after centrifugation at 5600 × g; (10) membrane – L binding substance collected and (11) membrane – L binding solution dissolved in organic solvent.