Figure 2
From: Structure, substrate recognition and reactivity of Leishmania major mevalonate kinase
The over-expression of Tb MK and Lm MK in procyclic-form T.brucei. TbMK and LmMK were cloned into the pLew82 vector and transformed into procyclic T. brucei to express ectopic C-terminal HA-tagged proteins under tetracycline control. Mid-log procyclic cells over-expressing Tb MK-HA (lane1), Lm MK-HA (lane 2) and wild type (lane 3) were washed, lysed and run on an SDS/10 % polyacrylamide gel and transferred to an ECL-Nylon membrane by Western blotting and detected with anti-HA antibodies. The expected molecular weight of both Tb MK-HA and Lm MK-HA is 36.9 kDa.